![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simiple, rapid, and highly sensitive fast microbore HPLC method with UV detection has been developed for the quantitation of coenzyme Q10 (Co-Q10) and related substances in raw material, pharmaceutical, and cosmetic formulations using a column with hybrid particles. Chromatographic conditions were: 30ºC column temperature, 1 µL injection volume at 275 nm. The Co-Q10 analysis was performed within 2.5 minutes with a flow rate of 0.3 mL/min. with a mobile phase consisted of 100% methanol, and the related substances were separated with a gradient elution based on methanol-water. The developed method was validated for selectivity, linearity, precision, accuracy, and robutness. The method was found to be suitable for the quality control of Co-Q10 in pharmaceutical and cosmetic products, as well as the stability indicating studies.
ACKNOWLEDGMENT
The authors wish to thank Instituto de Investigaciones Cardiologicas Dr.Taquini for providing the HPLC equipment and Dr Jorge Muse for assistance.
Notes
a According to USP 29 (15)
b Between Co-Q0 and DU
c between DU and Co-Q9
d between Co-Q9 and UB
e between UB and Co-Q10.
a Recovery mean values obtained from three individual samples on three different days.
RSD values in parenthesis.
a Upper and lower case letter represent high and low level of the variable, respectively.
Experimental design according to reference 17.
a Critical statistic (Ec = tc(p=0.05) √2 SD).
a Results are expressed as mean values (n = 3), RSD values in parenthesis.