Isotachophoresis of Chosen Biologically Active (E)-Azastilbenes

Abstract

Biological activity of (E)-azastilbenes and their numerous derivatives appears in antimicrobial and anticancer effects. They also show the behavior of liquid crystals and the ability of formation of complex compounds. In this work are presented the conditions of optimum separation and determination of chosen isomers of (E)-azastilbenes by the isotachophoretic method. In the process of optimization, the length of steps of analysis, intensity (it was justified by the fact of dependence of ion mobility on electric field intensity), and pH of solutions of electrolytes and samples were changed. A new terminating electrolyte was used for the determinations. By a process of trial and error it was proven that optimum pH for analyzed isomers are 3.8. The shortest time of analysis was obtained for individual isomers. However, for mixtures of isomers the optimum time of analysis was twice as long.

Keywords:

• (E)-Azastilbenes
• Isomers
• Isotachophoresis
• Optimization
• Terminating

Notes

1-Staphylococcus aureus 209P FDA, 2-Streptococcus faecalis ATCC 8040, 3-Bacillus subtilis ATCC 1633, 4-Escherichia coli PZHO 26B6, 5-Klebsiella pneumoniae 231, 6-Pseudomonas aeruginosa 5 R1, 7-Candida albicanus PCM 1409 PZH, 8-Microsporum gypseum K₁, 9-Aspergillus fumigatus C1.

^a n = 5, the samples were analyzed twice.

^b The sample was enriched with 1.5 mL of a solution containing 1 mg·mL⁻¹ of examined ion, n = 5.

^c Correlation coefficient above 0.97.

^d Calculated from the limit of identification and coefficients of the calibration curve.