APPLICATION OF TWO DIMENSIONAL THIN LAYER CHROMATOGRAPHY PATTERN COMPARISON FOR FINGERPRINTING THE ACTIVE COMPOUNDS IN THE LEAVES OF VITEX TRIFOLIA LINN POSSESSING ANTI-TRACHEOSPASMOLYTIC ACTIVITY

Alfi Khatib Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, Selangor Darul Ehsan, Malaysia; Department of Food Science and Technology, Bogor Agricultural University, Bogor, IndonesiaCorrespondence[email protected]

Arie C. Hoek Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, RA Leiden, The Netherlands

Selamat Jinap Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, Selangor Darul Ehsan, Malaysia

Md. Zaidul Islam Sarker Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, Selangor Darul Ehsan, Malaysia

Irwandi Jaswir Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia

Robert Verpoorte Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, RA Leiden, The Netherlands

Abstract

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase.

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APPLICATION OF TWO DIMENSIONAL THIN LAYER CHROMATOGRAPHY PATTERN COMPARISON FOR FINGERPRINTING THE ACTIVE COMPOUNDS IN THE LEAVES OF VITEX TRIFOLIA LINN POSSESSING ANTI-TRACHEOSPASMOLYTIC ACTIVITY

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APPLICATION OF TWO DIMENSIONAL THIN LAYER CHROMATOGRAPHY PATTERN COMPARISON FOR FINGERPRINTING THE ACTIVE COMPOUNDS IN THE LEAVES OF VITEX TRIFOLIA LINN POSSESSING ANTI-TRACHEOSPASMOLYTIC ACTIVITY

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