86
Views
3
CrossRef citations to date
0
Altmetric
Original Articles

SIMULTANEOUS EXTRACTION AND SEPARATION OF MARTRINE, SOPHORIDINE AND SOPHOCARPINE FROM SOPHORA FLAVESCENS AIT BY RP-HPLC WITH ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY

&
Pages 259-269 | Published online: 29 Dec 2009
 

Abstract

The simultaneous extraction and separation of martrine, sophoridine and sophocarpine from Sophora flavescens Ait by RP-HPLC was developed by liquid-liquid extraction. The optimum extraction conditions were established using different extraction solvents, procedures and time. The extracts were separated and determined using a C18 column with methanol/water/diethylamine (55/45/0.10, v/v) as the mobile phase. Different preparative columns with different packing sizes were used to isolate the three compounds from the extracts, and the column with a 12 μm particle size showed better separation. The amounts and recovery of sophocarpine, sophoridine and martrine obtained were 0.081 mg/g, 0.031 mg/g and 0.051 mg/g, and 85.38%, 87.14% and 76.5%, respectively.

ACKNOWLEDGMENT

This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2009-0072787).

Notes

*not be detected.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.