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Abstract
—Matrix solid-phase dispersion (MSPD) extraction and HPLC have been used for determination of paeonol and ferulic acid in “Su-Xiao-Xin-Tong Dropping Pills” (SXDP). For extraction of these compounds, the type of sorbent, the type and volume of elution solvent were studied. The highest yields were obtained with a ratio 4:1 of C18 to sample and 10 mL methanol-water-glacial acetic acid (80:20:0.5, v/v). The chromatographic separation was achieved with methanol:water:glacial acetic acid (50:50:0.5, v/v) as mobile phase at a flow rate of 1.0 mL/min, a Dikma Diamonsi-C18 column (250 mm × 4.6 mm, 5 µm), and UV detection at 315 nm. Linearity (R2 > 0.999), intra- and inter-day (RSD < 2.0%) precision were determined. The linearities of paeonol and ferulic acid were in the range of 25–250 µg/mL and 2.5–25 µg/mL, respectively. The limit of detection was found to be 0.080 µg/mL for paeonol and 0.030 µg/mL for ferulic acid. The mean recoveries for paeonol and ferulic acid were 94.5 and 93.3%, respectively. It is a simple and sensitive analytical method with good accuracy and reproducibility.
ACKNOWLEDGMENTS
This work was financially supported by the Research Project of Administration of Traditional Chinese Medicine of Heilongjiang Province, China (Grant No. ZHY 10-W69).
Notes
a Mean = Average of three determination; S.D. = Standard deviation. b n = Number of determination. c RSD ˭ Relative standard deviation.
a n = Number of determination; b k = Asymmetry factor; c RSD˭Relative standard deviation.
