EVALUATION OF NOVEL SOLID-PHASE EXTRACTION MICROSPHERES PREPARED BY A SURFACE IMPRINTING TECHNIQUE AND ITS APPLICATION TO THE SEPARATION OF DIETHYLSTILBESTROL FROM CHICKEN SAMPLES

Abstract

A novel molecular imprinted solid-phase extraction technique was developed by using microspheres prepared by modifying the silica through a sol-gel process with a layer of homogeneous and compact molecularly imprinted polymer (MIP), which was synthesized with 3-aminopropyltriethoxysilane and phenyltrimethoxysilane as functional monomers, diethylstilbestrol (DES) as the template, and tetraethoxysilane as the cross-linker. The saturated adsorption capacity of the MIP under the condition of continuous loading of target solution was 0.213 mg g⁻¹, and the imprinting factor reached 6.0. The selectivity factor of the MIPs for bisphenol A and 4-tert-butylphenol were 3.5 and 9.6, respectively. The molecular imprinted solid-phase extraction (MISPE) is a viable and efficient technique for extraction of DES from chicken samples with a recovery ofmore than 85%. The limit of detection (LOD) was determined to be 8 ng g⁻¹.

Keywords:

• diethylstilbestrol
• molecularly imprinted polymer
• sol-gel
• solid-phase extraction microsphere

ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China (No. 20875034) and the Science and Technology Plan Projects of Guangdong Province (No. 2008B030303073).

Notes

S = elution (template)/elution(analogue).