![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simple HPLC-UV method was developed and validated for the analysis of lysergol, its stress degradation profile, and pH stability studies. Instrument used was Agilent HPLC 1120 autosampler with a C18 reversed phase column and a mobile phase comprised of methanol and water (1% acetic acid) with isocratic flow. Flow rate was kept constant at 1 mL/min throughout the experiment and elution was monitored at 225 nm using UV detector. The calibration plot showed good linear relationship with r 2 = 0.9997. A well resolved single peak was observed at retention time 2.577 minute with asymmetry <1.0. The limit of detection (1.3 µg/mL), limit of quantitation (4.0 µg/mL), RSD (%) (<2.0) and capacity factor (k' = 1.577) were found to be within the acceptable limits. The stress degradation studies revealed lysergol recovery of 99.0% (wet heat induced), 65.9% (base induced), 63.7% (acid induced), 69.7% (H 2 O 2 induced), and 41.7% (UV light induced); whereas, complete drug degradation occurred at dry heat and photochemical induced degradation. From the pH rate profile studies, pH of maximum stability for lysergol was found to be 2.0.
ACKNOWLEDGMENT
S. Javed wishes to thank the Indian Council of Medical Research (ICMR), New Delhi, India, for providing financial assistance in the form of a Senior Research Fellowship. The authors are also thankful to The Chemical Resources, Panchkula, Haryana, India, for providing the gift ample of lysergol (97%) and Jamia Hamdard University for providing the scientific environment and ambience.
