DETERMINATION OF ATRACTYLODIN IN RAT PLASMA BY HPLC-UV METHOD AND ITS APPLICATION TO A PHARMACOKINETIC STUDY

Abstract

A simple and rapid method for quantification of atractylodin in rat plasma was developed by high-performance liquid chromatography with UV detection for the first time. The mobile phase consisted of acetonitrile and water (76:24, v/v). The wavelength was set at 340 nm. The calibration curve ranged from 0.025–5.0 µg/mL. Both accuracy and precision were satisfactory. This new method was applied to pharmacokinetics of atractylodin after oral administration of 30 g/Kg Atractylodis Rhizoma and intravenous administration of 2 mg/Kg of single atractylodin to rats. For oral administration, the main pharmacokinetic parameters C_max, T_max, T_0.5, and k_e were 1.93 ± 0.9 mg/L, 2.25 ± 0.5 hr, 8.08 ± 1.3 hr, and 0.09 ± 0.02/hr, respectively. For intravenous administration, T_0.5, Ke, Vc, and CL were 3.30 ± 0.38 hr, 0.21 ± 0.02/hr, 31.7 ± 5.8 L/kg, and 6.59 ± 1.1 L/h/kg, respectively.

Keywords:

• atractylodin
• atractylodis
• pharmacokinetics
• rat plasma
• rhizoma
• RP-HPLC

ACKNOWLEDGMENT

We would like to thank the Science and Technology of China (2009ZX09502-021 and 2009ZX09308-003) for financial support for this research.