Abstract
Lignans and flavonoids were isolated from Cuscuta racemosa Mart. & Humb (Convolvulaceae) by Droplet Counter-Current Chromatography (DCCC). The solvent system was composed of hexane/ethyl acetate/methanol/water (1:2:1:1, v/v). In this experiment it was possible to isolate six substances: 9α-hydroxysesamin, 9β-hydroxysesamin, kaempherol, acuminatolide, quercetin, and pinoresinol. The chemical structures of the six substances were elucidated by NMR and GC-MS.
ACKNOWLEDGMENT
The authors wish to thank the Analytical Center Laboratory of Far-Manguinhos, Fiocruz (Manguinhos, Rio de Janeiro, Brazil), CNPq and FAPERJ for financial support and Mr. John Marr Ditty for his contributions.