ISOLATION AND QUANTIFICATION OF VANILLIN THROUGH FLASH & HPTLC CHROMATOGRAPHIC TECHNIQUES FROM DECALEPIS HAMILTONII WIGHT AND ARN ROOT AND THEIR ANTIOXIDANT STUDIES

Abstract

Decalepis hamiltonii Wight and Arn (family: Asclepiadaceae) roots are widely used in folk and traditional medicine in India. In the present study, we isolated vanillin through flash chromatography, and performed quantification and validation through HPTLC in the root of D. hamiltonii. The developed method was also validated for accuracy, precision, robustness, limit of detection and quantification (LOD and LOQ), repeatability, and recovery according to ICH guidelines. Separation and quantification was achieved using toluene:ethyl acetate (9.0:1.0 v/v) as a binary mobile phase on precoated silica gel 60 F₂₅₄ aluminum plates on HPTLC. The HPTLC densitometry was carried out at a wavelength of 254 nm in the absorption-reflectance mode. The antioxidant potential of D. hamiltonii root sample was also studied by the 2,2-Diphenyl-2-picryllhydrazyl (DPPH) method. Aqueous plant extract showed 54.67% radical scavenging ability at 0.1 mg/mL concentration.

Keywords:

• antioxidant
• Decalepis hamiltonii
• flash chromatography
• HPTLC
• quantification
• vanillin

ACKNOWLEDGMENT

The authors are thankful to the Director, National Botanical Research Institute, Lucknow, India, for providing all the facilities to conduct the research work.

Notes

^a Relative Standard Deviation, RSD (%), n = 3).

RSD, Relative Standard Deviation (%), (n = 3).

Mean ± Standard deviation SD (n = 3).