SIMULTANEOUS EFFECT OF ORGANIC MODIFIER AND PHYSICOCHEMICAL PARAMETERS OF SOME STEROIDAL DRUGS ON THEIR RETENTION ON A NARROW-BORE C₁ COLUMN. PART 2

Abstract

The retention of 13 steroidal drugs was determined on a narrow-bore C1 column; the mobile phases being mixtures of water-methanol mixtures and the retention factor (k) was calculated for each mobile phase and drug. The relationships among the chromatographic parameters, mobile phase composition, and physicochemical parameters of solutes were elucidated by principal component analysis followed by two-dimensional non-linear mapping and varimax rotation around two axes. Computations indicated that the binding of steroidal drugs to the surface of the stationary phase is of mixed character: electrostatic and polar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exerts a considerable influence on the retention behavior. The comparison of results with results obtained by employing other sets of steroidal drugs indicated the marked effect of the character of substituents on the results of QSRR computations.

Keywords:

• narrow-bore column
• organic malifiers
• physicichemical parameters
• steroids

Notes

Note: For symbols see the Experimental section.

Part 1 was published in Chemom. Intell. Lab. System. 2004, 72, 269–275.