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Abstract
Echinacoside and acteoside were isolated and purified from Cistanche tubulosa (Schrenk) Wight by one step of high-speed counter-current chromatography for the first time using a solvent system of ethyl acetate-n-butanol-glacial acetic acid-water (1:1.2:0.2:2, v/v/v/v). The experiment yielded 16.9 mg of echinacoside and 5.1 mg of acteoside from 220 mg of Cistanche tubulosa (Schrenk) Wight extract. The purity of the two compounds was 99.14% and 95.04%, respectively, as determined by HPLC. Their structures were identified by MS, UV, IR, and NMR analysis. Such a simple and effective method was fairly useful to prepare pure compounds as reference substances for related study on Cistanche tubulosa (Schrenk) Wight.
ACKNOWLEDGMENT
This work was supported by the International Scientific and Technological Cooperation Program of China (No: 2009DFA31230) and the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No: 2010B090400533).
Notes
Note. K, solute concentration in the upper mobile phase divided by that in the lower stationary phase.