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Abstract
Paeoniflorin and albiflorin were isolated and purified from Radix Paeoniae Rubra by high-speed counter-current chromatography with a solvent system of ethyl acetate-n-butanol-water (3:2.5:5, v/v/v) in one step. From 100 mg of the n-butanol extract of Radix Paeoniae Rubra, 10 mg of paeoniflorin and 4.3 mg of albiflorin were obtained with purity of 98.75% and 95.03%, as determined by HPLC. Their structures were identified by MS, UV, and NMR analysis. In this study, a rapid method for isolation and purification of the two major compounds from Radix Paeoniae Rubra crude extract was established.
ACKNOWLEDGMENT
This work was supported by the International Scientific and Technological Cooperation Program of China (No: 2009DFA31230) and the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No: 2010B090400533).
Notes
K, solute concentration in the upper mobile phase divided by that in the lower stationary phase.