Abstract
A chromatographic-densitometric method was developed for determination of tolperisone hydrochloride and its impurities. Silica gel TLCF 254 plates were used as the stationery phase and cyclohexane-1,4 dioxane-isopropanol–ethanol-glacial acetic acid (16:0.5:1:4:0.6 v/v/v) and n-butanol-isopropanol-water-glacial acetic acid (10:7:8:2 v/v/v/v) as the mobile phases. Densitometric measurements were done at 260 nm for tolperisone hydrochloride and 4-methylpropiophenone and at 570 nm for piperidine hydrochloride. The method is specific, sensitive, precise, and has a broad range of linearity, with potential application in pharmaceutical analysis.
Notes
P, peak surface area; m, mass [µg per band]; a, slope of regression line; b, y-intercept, RSD, relative standard deviation; xsr, mean.
Sd, standard deviation; RSD, relative standard deviation.
Sd, standard deviation; RSD, relative standard deviation.