HPLC DETERMINATION OF DRDE-07 IN MICE PLASMA: PROBABLE ANTIDOTE FOR SULFUR MUSTARD TOXICITY

Abstract

DRDE-07 is a new synthetic compound found to be the most prophylactic agent against sulfur mustard toxicity. In this study, the development and validation of a high-performance liquid chromatography (HPLC) assay for determination of DRDE-07 concentration in mice plasma for pharmacokinetic studies is described. A simple protein precipitation procedure by ice cold acetone was applied to extract DRDE-07 from plasma. The intra-day and inter-day precision of DRDE-07 ranged from 1.75% to 4.38% and from 1.07% to 3.21%, respectively. Similarly, the intra-day and inter-day accuracy of DRDE-07 ranged from 95.56% to 100.07% and from 95.69% to 101.02%, respectively. The extraction recovery of DRDE-07 was 96.87 ± 1.89% at 1.0 µg/ml (n = 3), 96.43 ± 1.41% at 10 µg/mL (n = 3), and 88.26 ± 1.49% at 100 µg/mL (n = b3). Calibration curves of DRDDE-07 were linear in the concentration range of 1–100 µg/mL in plasma. The limit of quantification was 1 µg/mL. This method was applied to determine DRDE-07 concentration in mice plasma samples for a pharmacokinetic study.

Keywords:

• antidote
• DRDE-07
• HPLC
• pharmacokinetics
• sulfur mustard
• validation

Notes

*Not determined.