CHIRAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF BEVANTOLOL HYDROCHLORIDE

Abstract

A new liquid chromatograpic method has been developed for the chiral separation of the enantiomers of bevantolol hydrochloride. The enantiomers were separated by a Chiralpak IB (250 × 4.6 mm, 5 µm) with a binary solvent mixture of acetonitrile—20 mmol · L⁻¹ KH₂PO₄ (25/75, v/v; pH 4.5)—at 25°C and detected at 259 nm, with a flow rate of 0.8 mL · min⁻¹. The resolution was 3.90. The effects that organic modifier, type of buffer, mobile phase pH, column temperature, and flow rate had on the enantioselectivity of enantiomers were evaluated. And the structures of the enantiomers of bevantolol hydrochloride were determined.

Keywords:

• analysis
• bevantolol hydrochloride
• Chiralpak IB
• chiral separation
• HPLC
• enantiomer

Notes

Chromatographic condition: the column temperature was at 25°C, and the flow rate was 0.8 mL · min⁻¹; mobile phase was acetonitrile +20 mM KH₂PO₄ buffer (pH 4.5).

Chromatographic condition: mobile phase was acetonitrile +20 mM buffer, the column temperature was at 25°C, and the flow rate was 0.8 mL · min⁻¹.

Chromatographic condition: mobile phase was acetonitrile +20 mM KH₂PO₄ buffer, the column temperature was at 25°C, and the flow rate was 0.8 mL · min⁻¹.

Chromatographic condition: mobile phase was acetonitrile—20 mmol · L⁻¹ KH₂PO₄ (25/75, v/v; pH 4.5)—the flow rate was 0.8 mL · min⁻¹.

Chromatographic condition: mobile phase was acetonitrile—20 mmol · L⁻¹ KH₂PO₄ (25/75, v/v; pH 4.5)—at 25°C.

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