USE OF TLC FOR THE QUANTITATIVE DETERMINATION OF ACETYLSALICYLIC ACID, CAFFEINE, AND ETHOXYBENZAMIDE IN COMBINED TABLETS

Abstract

Adsorption thin layer chromatography (NP-TLC) with densitometry has been established for the identification and the quantification of acetylsalicylic acid, caffeine, and ethoxybenzamide in combined tablets. UV densitometry was performed in absorbance mode at 200 nm, 275 nm, and 300 nm for acetylsalicylic acid, caffeine, and o-ethoxybenzamide, respectively. The presented method was validated by specificity, range, linearity, accuracy, precision, detection limit, quantitative limit, and robustness. It could be said that the NP-TLC-densitometric method is suitable for the routine analysis of acetylsalicylic acid, caffeine, and ethoxybenzamide in combined tablets in quantity control laboratories.

Keywords:

• acetylsalicylic acid
• caffeine
• densitometry
• ethoxybenzamide pharmaceutical analysis
• quantitative analysis
• spectrodensitometry
• TLC

ACKNOWLEDGMENT

This research was financed by the Medical University of Silesia as part of the statutory research projects in 2012 and 2013.

Notes

^a ASA – acetylsalicylic acid, EB – o-ethoxybenzamide, C – caffeine.

^a ASA – acetylsalicylic acid, C – caffeine.

^a A – peak area (AU), x – amount (µg · spot⁻¹) of drug analyzed, r- correlation coefficient, R – recovery (%), CV – coefficient of variation (%).

^a A – peak area (AU), x – amount (µg · spot⁻¹) of drug analyzed, r- correlation coefficient, R – recovery (%), CV – coefficient of variation (%).

^a A – peak area (AU), x – amount (µg · spot⁻¹) of drug analyzed, r- correlation coefficient, R – recovery (%), CV – coefficient of variation (%).

^a ASA – acetylsalicylic acid, EB – o-ethoxybenzamide, C – caffeine.

^a ASA – acetylsalicylic acid, C – caffeine.