OPTIMIZATION OF SOME VARIABLES OF ON-LINE INJECTION IN PRESSURIZED PLANAR ELECTROCHROMATOGRAPHY

Abstract

Pressurized planar electrochromatography (PPEC) is a separating technique, in which an electric field is applied to force movement of the mobile phase through a porous media (electroosmotic effect). High separation efficiency, fast separation, and changes in separation selectivity in comparison to liquid chromatography, especially to thin-layer chromatography (planar chromatography, TLC) are features of this technique. Our group has recently introduced the prototype device, which enables simultaneous on-line injection of six samples on chromatographic plate in a stream of the mobile phase. In this paper influence of some operating variables of on-line injection mode on efficiency of PPEC separation system is shown and discussed.

Keywords:

• acetaminophen
• on-line sample injection
• optimization of injection variables
• pressurized planar electrochromatography (PPEC)
• reversed phase PPEC
• separation efficiency

Notes

ACE, acetanilide; PAR, acetaminophen; z, migration distance of solute zones; H, plate height.

ACE, acetanilide; PAR, acetaminophen; z, migration distance of solute zones; R_S, resolution; A_S, peak asymmetry at W_0.1; H, plate height.