Abstract
A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of epothilones from strain S. cellulosum Fim0810 fermentation broth. Epothilone A and B components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of n-hexane-ethyl–acetate–methanol–water (7:7:5:5 by volume). In a single operation, 100 mg of the crude extract was separated to yield 22.8 mg of Epothilone A and 38.4 mg of Epothilone B with the purity of 97.2% and 96.5%, respectively. The HSCCC fractions were determined by high-performance liquid chromatography (HPLC), UV, ESI–MS, and 1H NMR analysis. This technique should also be applicable to the preparation of epothilone analogs from Sorangium cellulosum fermentation broth.
Acknowledgment
H. J. Yang and Z. L. Zhang contributed equally to this report.
Notes
Color versions of one or more of the figures in the article can be found online at www.tandfonline.com/ljlc.