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Abstract
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q m = 29.15 mg PCI/mL of adsorbent and K d = 0.0145 mg de pCI/mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k 1 = 0.0067 mL/mg s.