A Simple and Rapid HPLC Method for the Direct Determination of Residual Monomers Released From Dental Polymeric Materials in Blood Serum and Urine

Abstract

Bisphenol-A (BPA), Triethylene glycol dimethacrylate (TEGDMA), Bisphenol A glycerolatedimethacrylate (Bis-GMA), and Urethane dimethacrylate (UDMA) are organic monomers that can be released from dental composites and, depending on their concentration in biological fluids, can cause adverse reactions and toxic or other effects. Herein, a simple and rapid method has been developed for the simultaneous determination of the aforementioned monomers in human blood serum and urine. Chromatographic analysis was performed isocratically on a Perfect Sil 120 ODS-2 analytical column (250 mm × 4.0 mm, 5 µm) with CH₃CN:H₂O, 70:30%, v/v as mobile phase within 6 min. The developed method was validated in terms of selectivity, linearity, accuracy, precision, and sensitivity in spiked matrices. Repeatability and between-day precision over a period of five days revealed Relative Standard Deviation (RSD) values lower than 13.1% for blood serum samples and lower than 6.6% for urine samples. Recovery of the examined analytes ranged from 92.6% to 106.1% in blood serum samples and from 95.0% to 106.9% in urine samples. The stability of the four monomers in blood serum and urine samples was studied in terms of long-term storage (at 4°C and −18°C), short-term storage (room temperature), and during sequential freeze-thaw cycles.

Keywords:

• bisphenol-A (BPA)
• bisphenol A glycerolatedimethacrylate (BIS-GMA)
• blood serum
• dental composites
• dimethacrylate monomers
• HPLC
• triethylene glycol dimethacrylate (TEGDMA)
• urethane dimethacrylate (UDMA)
• urine

Notes

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