Effects of β-glucanase-Immobilized Silica on Hydrolysis of Polysaccharides in Chamaecyparis obtusa Residues

Abstract

Enzymatic hydrolysis, which is one of the steps in the production of bioethanol, is a major process in the degradation of polysaccharides. Enzyme-immobilized technology has been developed because enzymes can be contaminated easily and are difficult to reuse. In this study, β-glucanase was immobilized on silica, and the effects of the enzyme-silica size, time, temperature, and amount of enzyme-silica on the hydrolysis of polysaccharides in the Chamaecyparis obtusa residue were studied. The enzyme-silica size, time range, temperature, and amount of enzyme-silica studied ranged from 10 to 63 µm, 2 to 12 hr, 25 to 45°C, and 0.05 to 0.25 g, respectively. The optimal enzyme-silica size, time range, temperature, and amount of β-glucanase immobilized on enzyme-silica for the hydrolysis of polysaccharides were found to be 10–25 µm silica, 10 hr, 40°C, and 0.15 g, respectively. The enzyme could not be used at temperatures higher than 40°C due to denaturation. Under the optimal conditions, the glucose yield was 12.77 mg/mL.

Keywords:

• β-glucanase
• Chamaecyparis obtuse
• hydrolysis
• immobilization
• polysaccharides
• silica

Notes

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Additional information

Notes on contributors

Yu Ri Lee

Yu Ri Lee and Baokun Tang contributed equally to this work and should be considered co-first authors.