372
Views
28
CrossRef citations to date
0
Altmetric
Original Articles

C18-Functionalized Magnetic Silica Nanoparticles for Solid Phase Extraction of Microcystin-LR in Reservoir Water Samples Followed by HPLC-DAD Determination

, , , , &
 

Abstract

In this study, C18-functionalized magnetic silica nanoparticle (Fe3O4@SiO2@C18 MNPs) based magnetic solid phase extraction (MSPE) was successfully developed for the determination of microcystin-LR (MC-LR) in reservoir water samples followed by high performance liquid chromatography–diode array detection (HPLC–DAD). After the extraction, the adsorbent can be conveniently and rapidly separated from aqueous samples by an external magnet. The main factors influencing the extraction efficiency including the amount of the MNPs, the extraction time, the pH of sample solution, and desorption conditions were optimized to obtain high recoveries and extraction efficiency. High enrichment factor 500 was attained. Under the optimized experimental conditions, the calibration curve of MC-LR was linear in the range of 0.1–10.0 µg/L with correlation coefficients (r2) 0.9996. Limit of detection (LOD, S/N = 3) of the method was 0.056 µg/L. The developed method was successfully applied to the determination of MC-LR in reservoir water samples. The method recoveries were obtained ranging from 73.3–104% for three spiked concentrations, with the relative standard deviations (RSD) of 2.90–4.30%. The developed Fe3O4@SiO2@C18 MNPs-based MSPE coupled with HPLC–DAD demonstrated excellent sensitivity and repeatability, simplicity, rapidity, and ease of operation, as well as practical applicability.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.