Dissipation Kinetics of Cyazofamid in Water

Abstract

A reliable and sensitive method has been developed and validated for the determination of cyazofamid in water using reverse phase HPLC (RP-HPLC). The analytical procedure involves fortification of water at two doses, liquid–liquid partition with chloroform, separation on Discovery C-18 column using mobile phase acetonitrile: water (60:40 v/v) and detection at 279 nm. Calibration curves for quantification were linear over the dynamic range of 0.01–10.0 µg mL⁻¹ concentrations with the correlation coefficients (R²) greater than 0.99 and percent relative standard deviation [RSD (%) was 1.72. Degradation pattern indicated correspondence to monophasic first order kinetics in water with half-life values 3.92 and 4.25 days. The degradation rate was almost same at both treatments. The average recoveries of cyazofamid from water fortified at 0.01–5.0 µg mL⁻¹ ranged from 90.4–96.6%. The limit of quantitation and limit of detection of analyte were 0.01 and 0.006 µg mL⁻¹, respectively.

Keywords:

• cyazofamid
• dissipation
• kinetics
• RP-HPLC
• water

Acknowledgments

The authors are thankful to M/s Ishihara Sangyo Kaisha (ISK) Biosciences Corporation, Japan (through United Phosphorus Ltd, India) for providing analytical cyazofamid for research purposes. The authors also acknowledge to G. B. Pant University for providing necessary facilities for research work.