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Abstract
A method for quantitative analysis of pectin in lozenges is reported. The separation was achieved using size exclusion chromatography. While pectin has no chromophore, to detect the separated pectins, an on-column derivatization with phenylboronic added as an additive in the mobile phase followed by indirect photometric detection was employed. The mobile phase, pH, and concentration of the additive were investigated to develop the optimal method with good linearity (R2 = 0.999). The method was applied to the determination of quantity of pectins in commercial lozenges.