Detection reagents used in on-plate identification of amino acids by thin layer chromatography: A review

Abstract

This review describes the use of different modes of planar chromatographic techniques such as conventional thin layer chromatography (TLC) and high performance TLC (HPTLC) for separation and analysis of essential and non- essential amino acids and their derivatives. The use of new developed spray reagents and the ninhydrin itself in the analysis and separation of amino acids by TLC have been briefly encapsulated in tabular form.

Graphical Abstract

Keywords:

• Amino acids
• green solvents
• HPTLC
• ninhydrin
• RF value; thin layer chromatography (TLC)

Acknowledgments

The authors are highly grateful to HOD department of Chemistry, School of sciences for providing facilities and guidance. One of the authors (Qasimullah) is also highly thankful to the University Grant Commission for funding through Minor Research Project.