Quality evaluation of triterpenoids in Ganoderma and related species by the quantitative analysis of multi-components by single marker method

Abstract

Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method for the determination of triterpenoids and distinction of Ganoderma and related species. Methods: Ten ganoderma triterpenoids were determined by HPLC in addition to a QAMS method. On the basis of QAMS, principal component analysis (PCA) and cluster analysis (CA) were used to distinguish Ganoderma samples.

Results: Ten types of ganoderma triterpenoids had good linearity of regression coefficients (R² ≥ 0.9992). Relative correction factors (RCFs) of lucideric acid C, lucideric acid LM₁, ganoderic acid G, lucideric acid B, lucideric acid A, ganoderenic acid D, ganoderic acid D, lucideric acid D and ganoderic acid F with reference to ganoderic acid A were 0.929, 1.011, 0.608, 1.041, 0.997, 2.464, 0.891, 0.826 and 0.722, respectively. Ten types of ganoderma triterpenoids in twenty-nine batches of Ganoderma and related species were determined by QAMS. Nineteen batches of samples containing ganoderma acid A were roughly divided into three categories by PCA and CA.

Conclusion: The developed QAMS method, in combination with PCA and CA, served as an accurate method for the rapid evaluation and authenticity identification of Ganoderma.

GRAPHICAL ABSTRACT

KEYWORDS:

• Ganoderma triterpenoids
• HPLC
• principal component analysis
• QAMS
• quality control

Additional information

Funding

This work was supported by the National Natural Science Foundation of China under Grant No. 81603249 and the Scientific Research Project of the Science Technology Department of Zhejiang Province under Grant No. 2016C33076.