Food, drug and model mix analysis by organic monolithic columns based on heterocyclic monomers

Abstract

This paper presents examples of separation on monolithic columns prepared by two different methods: copolymerization and post-modification. In the first method, monoliths were prepared from styrene, divinylbenzene, and heterocyclic nitrogen-containing monomers. In the second, a styrene/divinylbenzene/4-vinylbenzyl chloride copolymer was obtained and in situ modified with 1-methylimidazole. It has been shown that monolithic columns are capable of separating macromolecules and low-molecular compounds. A comparison was made between a styrene/divinylbenzene/1-vinylimidazole copolymer column and a ProSwift™ RP-3U monolithic column. It has been established that a monolithic column based on 1-vinylimidazole is capable of separating proteins and low molecular weight test substances. However, the ProSwift™ RP-3U column is only capable of separating proteins. Separation examples are given: a test mixture of proteins, natural milk proteins, carbohydrates and drugs on a column with a sorbent based on styrene/divinylbenzene. A modified column was used to study the content of salicylic acid in medicinal preparations containing acetylsalicylic acid.

Graphical Abstract

Keywords:

• HPLC
• macromolecules separating
• mixed-mode chromatography
• monolithic columns
• porous polymer

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported Ministry of Science and Higher Education of the Russian Federation within the governmental order for Boreskov Institute of Catalysis (project АААА-А21-121011390053-4).