Countercurrent Chromatography with a New Centrifugal Partition Chromatographic System

Abstract

Efficiency and resolution have been studied with a new centrifugal partition chromatograph, the series 1000 HPCPC(tm) system, classified by Y. Ito¹ as a HSES non-planetary with rotary seals. It has been shown that, in the range 1-10 ml/min, the efficiency is not directly dependent upon the flow rate, but increases markedly with the volume ratio of the mobile phase in the column. For each flow rate there is a minimum value for the volume of the mobile phase, and the relationship is linear; working within these conditions, changing the flow rate and the volume of the mobile phase leads to an efficiency which increases with the flow rate from 1 to 20 ml/min. This increase in efficiency, which goes through a decrease of the ratio of the stationary phase in the column, results in resolution for two peaks which is roughly constant over a broad range of flow rates.