Synthetic Peptide Purification in The Multi-Coil Countercurrent Chromatograph

Abstract

The multi-coil countercurrent chromatograph (MC-CCC) is used for preparative separations of various synthetic peptides. For peptides that are water insoluble and that have a high partitioning in the upper phase of n-butanol. acetic acid and water, the use of the more hydro phobic solvent system comprised of chloroform, acetic acid and water provides purification. An alternative two-phase solvent system made up of equal volumes of three components such as ethyl acetate, acetonitrile and an aqueous acid or salt solution is proposed for peptide separation as well.