Semipreparative Separation of Alkaloids from Cephalotaxus Fqrtunei Hook. F. by High-Speed Countercurrent Chromatography

Abstract

High-speed countercurrent chromatography was applied to the separation of harringtonine, isoharringtonine and homoharringtonine from Cephalotaxus fortunei Hook, f. The separation was performed with a two-phase solvent system composed of chloroform-0.07M sodium phosphate-0.04M citric acid buffer solution (pH 5.0). The fractionated components were identified with authentic pure compounds on TLC and PPC. The peak fraction of each component was analyzed with a mass spectrometer for structure identification. The results indicate that the method is suitable for semipreparative separations of these alkaloids.