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Abstract
A method to analyze parent atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine) and its major metabolites in soils by reverse-phase high performance thin layer chromatography (RP-HPTLC) is described. Atrazine is extracted with methanol, filtered, concentrated by evaporation and chromatographed on pre-coated RP-HPTLC plates. The concentration of atrazine is determined via densitometry. Additional clean-up of the extract is not required. Recovery of atrazine from fortified samples ranged from 87-97%. The detection limit of the method is 20 nanograms.