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Abstract
Procedures for isolation and purification of individual gangliosides from bovine brain, porcine brain, bovine omentum and porcine omentum have been developed yielding high quality products with better than 98% purity.
Bovine and porcine brain gangliosides yielded different patterns and concentrations of individual gangliosides. Contrary to bovine brain gangliosides, porcine brain showed an additional band corresponding to an appreciable quantity of GD3.
The major difference in ganglioside composition was noted between brain and omentum. Significant variations in types and composition of individual gangliosides were observed in the omentum tissue. Presence of double and triple TLC bands indicating various fatty acids on the ganglioside molecules were characteristic features of the omentum tissue which were not observed in the brain gangliosides.