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Abstract
A method for the determination of stevioside or rebaudioside A in beverages by TLC is described. The natural sweeteners are determined directly at concentrations of 100-200 ppm; below 100 ppm, the sample is purified by a reversed phase silica gel 60 silanized column chromatographic procedure. Quantification by scanning densitometry is carried out after TLC on C-18 preadsorbent layers and detection with anisaldehyde-sulfuric acid reagent. Recoveries from various beverages spiked at 10-200 ppm ranged from 81.8 to 111.0% (stevioside) 87.5 to 111.6% (rebaudioside A). TLC on HP silica gel allows separation of stevioside and rebaudioside A from each other, qualitative identification of the sweeteners, and rapid screening of beverages for their presence.