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Abstract
A rapid solid-phase extraction procedure utilizing C18 reverse-phase mini-columns has been established for dopamine, 3,4-dihydroxyphenylacetic acid, serotonin, and 5-hydroxyindoleacetic acid in tissue perfusate samples. The method rapidly separates the analytes from protein in albumin-containing tissue perfusates. The analytes which are recovered in high yield are then determined by high-performance liquid chromatography with electrochemical detection. The mean interassay coefficient of variation for the recovery of dopamine, 3, 4-dihydroxyphenylacetic acid, serotonin and 5-hydroxyindoleacetic acid was 12, 7, 13 and 8 percent, respectively.