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Original Articles

High-Performance Liquid Chromatographic Determination of Ethylenethiourea in Food

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Pages 525-534 | Published online: 23 Sep 2006
 

Abstract

An HPLC procedure was developed for the determination of ethylenethiourea (ETU) in peels of potatoes, tomatoes and apples after application of mancozeb suspension (ethyl-enebisdithiocarbamate fungicide). The analytical procedure consisted of a single extraction step with methanol and ETU was eluated from SEPARON CN column. Mobile phase was 4 % methanol in chloroform cyclohexane (1:1). The level of ethylenthiourea after application of mancozeb decreased slowly, with a half time six days in each case.

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