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Original Articles

Chromatographic and Spectroscopic Analysis of Globotriaosyl Ceramide from Bovine Spermatozoa

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Pages 1621-1638 | Published online: 23 Sep 2006
 

Abstract

The isolation and characterization of globotriaosyl ceramide from bovine spermatozoa is presented. Analysis of Gb3 by high-resolution proton nuclear magnetic resonance (1H-NMR), fast atom bombardment mass spectrometry (FAB-MS), mobility on HFTLC, and HPTLC-immunostaining; GLC/MS analysis of the partially methylated alditol acetates of the sugar units, and GLC analysis of the sphingoid bases and fatty acids present in the ceramide moietv. resulted in the characterization of mis major glycosphingolipid as Gal(αl-4)Gal(βl-4)Glc(β1-1)Cer.

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