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Abstract
A method for the determination of N-vinyl-2-pyrrolidinone (NVP) in both rat and dog plasma is described. The method involves the addition of an internal standard, a solution containing sodium lauryl sulfate, and an ultrafiltration step through a micropartition system which is subjected to centrifugation at 2000 × g for 15 minutes. The ultrafiltrate is then analyzed by an HPLC system with UV detection at 235 nm. The assay is linear in the concentration range of (0.2–8.0 μg/mL) for rat plasma with a minimum sensitivity of 100 ng/mL and a day to day variation of 3.0%. In the case of dog plasma, the assay is linear in the concentration range of (0.05–4.0 μg/mL) with a minimum sensitivity of 50 ng/mL when 100 μL aliquots of plasma are extracted. Application of this method to the protein binding studies of NVP to both rat and dog plasma is illustrated.