Application of an N-Methylpyridinium Polymer Column to Chromatographic Investigation of Human Serum Albumin-Sodium Aurothiomalate Complex

Abstract

A column containing N-methylpyridinium polymer was used in the chromatographic investigation of complex of human serum albumin and sodium aurothiomalate. The serum albumin-sodium aurothiomalate complex was separated from the components of serum albumin. The peak height of the complex increased with increasing the incubation time of albumin and sodium aurothiomalate, with a concomitant decrease of mercaptalbumin peak height. The nature of the mercaptalbumin participation in this complex formation was elucidated. The formation of the complex was observed in vitro, but not in the serum of a patient with rheumatoid arthritis receiving sodium aurothiomalate therapy.