Rapid Isolation of GM1, and GD1a from Bovine Brain Gangliosides by Propylamine and Q-Sepharose Column Chromatography

Abstract

Procedures for rapid isolation of GM1 and GD1a from bovine brain gangliosides have :een developed. The methods utilize conventional and over-load chromatography using propylamine and Q-Sepharose resins. Both procedures reguire low concentrations of eluting reagents without the use of salt for elution. Application of this method to small and large scale preparations have yielded GM1 and GD1a greater than 98% purity with recoveries better than 95%. The use of single solvent, elimination of salt throughout the entire chromatography, convenience and flexibility of the chromatographic parameters have made this technique suitable for rapid isolation of GM1, GD1a and the remaining bovine brain gangliosides.