The Influence of Packing Properties on the Isolation of Rutin from Plants and Drugs Using Solid Phase Extraction

Abstract

On the basis of sililca gel with strictly defined porosity the packings with different structure and coverage density of chemically bonded C₁₈ phase (CBP) for solid phase extraction (SPE) have been prepared. Home-made packings were compared with five commercially available SPE columns. All packings were applied for isolation of rutin from plant (Aesculus hippocastanwn) and drug (ESBERIVEN^R). Evaluation and choice of optimal SPE columns for isolation and purification of rutin from natural and pharmaceutic products were made by determination of adsorption isoterms and recoveries for standard solution and extract contained rutin.