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Abstract
A simple and reliable isocratic reversed-phase HPLC method for the determination of phenylacetic acid (PAA) in urine is described. After acid hydrolysis of its glutaminoconjugate, phenylacetic acid is extracted by diethylether; chromatography is followed by UV detection at 210 nm. This rapid method is quite specific and sufficiently sensitive. We found satisfactory linearity up to 200 mg/1 PAA urinary concentrations. Within-day and between-day assays showed variation coefficients below 10 %. Twenty-four hour urinary excretion of PAA might therefore be easily and routinely measured; it could represent a noninvasive and suitable biological marker in psychiatry.