Abstract
An HPLC procedure was developed for the determination of mancozeb on cucumber leaves after application of mancozeb suspension (ethylenebisdithiocarbamate fungicide). The analytical procedure consisted of a single extraction step with EDTA aqueous solution and sodium salt of ethylenebisdithiocarbamate was eluated from Separon NH2 column. Mobile phase was 5 % aceto-nitrile and 45 % methanol in aqueous solution of borate buffer at pH = 7.6. The level of mancozeb after application on leaves decreased slowly, with a half time 14 days.