Investigations into the Use of Poly (3-Methylpyrrole-4-Carboxylic Acid) Coated Silica as a Chromatographic Stationary Phase

H. Ge Material Technology Division, Singapore Institute of Standards and Industrial Research, 1 Science Park Drive, Singapore, 0511

K. J. Gilmore Intelligent Polymer Research Laboratory Chemistry DepartmentThe University of Wollongong, P. O. Box 1144, Wollongong, New South Wales, 2500, Australia

S. A. Ashraf Intelligent Polymer Research Laboratory Chemistry DepartmentThe University of Wollongong, P. O. Box 1144, Wollongong, New South Wales, 2500, Australia

C. O. Too Intelligent Polymer Research Laboratory Chemistry DepartmentThe University of Wollongong, P. O. Box 1144, Wollongong, New South Wales, 2500, Australia

G. G. Wallace Intelligent Polymer Research Laboratory Chemistry DepartmentThe University of Wollongong, P. O. Box 1144, Wollongong, New South Wales, 2500, Australia

Abstract

Poly (3-methylpyrrole-4-carboxylic acid) (PMPC) was coated on to a silica support for use as an HPLC stationary phase. Reversed phase anion exclusion and cation exchange chromatographic behaviour have been characterised using a group of test compounds. The precominant mode of interaction was found to be dependent on the eluent conditions. Separation of various compounds was carried out and the applicability of this stationary phase for protein secarations was investigated.

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Investigations into the Use of Poly (3-Methylpyrrole-4-Carboxylic Acid) Coated Silica as a Chromatographic Stationary Phase

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Investigations into the Use of Poly (3-Methylpyrrole-4-Carboxylic Acid) Coated Silica as a Chromatographic Stationary Phase

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