Purpald (4-Amino-3-hydrazino-5-mercapto-1, 2, 4-triazole) as a Reagent for Post-Column Derivatization of Neutral Monosaccharides in High Pressure Liquid Chromatography

Abstract

A comprehensive study of the reaction between Purpald (4-amino-3- hydrazino-5-mercapto-l, 2, 4-triazole) and eight neutral monosaccharides in order to establish the optimal conditions (wavelength, oxygen supply, temperature, time, pH and concentration range) was carried out. The reaction was applied to the post-column detection in HPLC of monosaccharides eluted with water from an ion-exchange column. The detection limits and linear determination ranges thus achieved make this reaction a valid alternative to the determination of carbohydrates.