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Abstract
A method for the determination of 3-amino-5-ethyl-6-methyl-pyridin-2-one in human plasma is described. Buffered (pH 8) plasma samples are extracted with 7.5% 2-propanol in chloroform after the addition of an internal standard. The extracts are evaporated to dryness and reconstituted in mobile phase prior to analysis via HPLC with fluorescence detection (Λex=314 nm, Λem=390 nm). The assay has been validated in the concentration range of 5–500 ng/ml when 1 ml samples are extracted. Application of the assay to the analysis of samples collected after oral dosing of a 2-pyridinone based specific HIV-1 reverse transcriptase inhibitor is demonstrated.