Liquid Chromatographic Analysis of Cocaine, Benzoylecgonine, Local Anaesthetic Agents and Some of Their Metabolites in Biological Fluids

Abstract

Cocaine and benzoylecgonine, other local anaesthetic agents (amides, esters) and some of their metabolites were determined in biological samples, after liquid-liquid or solid-liquid extraction, by high performance liquid chromatography, using a reversed-phase column, an appropriate internal standard, three mobile phases, with two flow rates, UV detection at 230 nm and 280 nm, and scanning UV spectra with photodiode array detector. Fluorimetric detection (ex. : 280 nm, em: 350 nm) was also available for characterizing the ester anaesthetic compounds. The method is selective and suitable for clinical pharmacology purposes as well as for forensic toxicology.