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Abstract
A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted to the Diagnostic Lab for assay of Vitamin K or possible coumarol. After evaluating normal phase and reverse phase Thin Layer Chromatography (TLC) plates and different solvents, reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and indication of Vitamin K. Gas Chromatography and Densitometry were used to guantitate the Vitamin K present in liver samples and confirmation of Vitamin K was done by Mass Spectroscopy.
