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Abstract
The application of panacyl bromide, a fluorescence labeling reagent for carboxylic acids, was examined. Fatty acids are separated by reversed-phase high-performance liquid chromatography. The derivatives of series of both saturated and unsaturated fatty acids (C10:0-C20:4) are simultaneously separated by a continuous gradient elution method using a methanol-based solvent containing acetonitrile. The quantitative detection is linear over a range of 10–500 pmol per injection. The method was demonstrated to be able to quantify free fatty acids in blood samples from normal subjects and diabetic patients.