Numerical Integration of Complex Chromatograms Using Fitted Gaussian Functions

Abstract

A microcomputer program is presented for the analysis and integration of peaks in chromatography using numerical gaussian fittings and repetitive peelings. The method is based on a IBM compatible PC microcomputer and Basic language. Data is transported to the computer from primary output curves of a chromatography device by a coordinate reader. This makes the system applicable to any chromatography apparatus. The program itself is adaptive to almost any IBM PC compatible microcomputer. The method was developed especially for the analysis of complex chromatograms with severely overlapped peaks. It was tested by using spectra of hemoglobin, obtained from cation exchange liquid chromatography.